DcR2 (TNFRSF10D)
(Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))
Type de proteíne
Recombinant
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette DcR2 protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Ala56-His211
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TRAIL, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14μg/ml determined by ELISA. The affinity constant of 0.135 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
TNFRSF10D
Origine: Humain
Hôte: Cellules d'insectes
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
DcR2 (TNFRSF10D)
(Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))
TNFRSF10D Protein, CD264 Protein, DCR2 Protein, TRAIL-R4 Protein, TRAILR4 Protein, TRUNDD Protein, TNF receptor superfamily member 10d Protein, tumor necrosis factor receptor superfamily member 10D Protein, TNFRSF10D Protein, LOC716901 Protein
Sujet
CD264, RSF10D, TRAILR4, DCR2, TRUNDD, TNFRSF10D,TNF-related apoptosis inducing ligand (TRAIL) is a potential antitumor protein known for its ability to selectively eliminate various types of tumor cells without exerting toxic effects in normal cells and tissues. TRAIL-R2/DR5 as well as TRAIL-R3/DcR1 and TRAIL-R4/DcR2 were significantly higher expressed in advanced tumour stages.
Poids moléculaire
19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.