FLT4 Protein (AA 25-776) (His-Avi Tag,Biotin)

N° du produit ABIN7275840

Détail du produit

Antigène: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Type de proteíne: Recombinant

Attributs du protein: AA 25-776

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FLT4 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human VEGF R3/FLT4 Protein

Séquence: Tyr25-Ile776

Attributs du produit: Recombinant Biotinylated Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF-C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 29.6ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Autre désignation: VEGF R3 (FLT4 Produits)

Synonymes: FLT41 Protein, LMPH1A Protein, PCL Protein, VEGFR3 Protein, AI323512 Protein, Chy Protein, Flt-4 Protein, VEGFR-3 Protein, Vegfr3 Protein, cb1059 Protein, fms related tyrosine kinase 4 Protein, FMS-like tyrosine kinase 4 Protein, fms-related tyrosine kinase 4 Protein, FLT4 Protein, Flt4 Protein, flt4 Protein

Sujet: Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

Poids moléculaire: 87.4 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling