MICA Protein (AA 24-308) (Fc Tag)

N° du produit ABIN7454187

Détail du produit

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Type de proteíne: Recombinant

Attributs du protein: AA 24-308

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette MICA protéine est marqué à la Fc Tag.

Fonction: Human MICA Protein

Séquence: Glu24-Gln308

Attributs du produit: Recombinant Human MICA Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu24-Gln308.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MICA, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45μg/ml determined by ELISA (QC Test). The affinity constant of 0.36 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: MICA (MICA Produits)

Synonymes: MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein

Sujet: MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..

Poids moléculaire: 59.64 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.

UniProt: Q96QC4

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis