Prothrombin Protein (AA 25-617) (His tag)

N° du produit ABIN7504306

Détail du produit

Antigène: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Type de proteíne: Recombinant

Attributs du protein: AA 25-617

Origine: Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette Prothrombin protéine est marqué à la His tag.

Fonction: Rat Coagulation Factor II Protein

Séquence: Gln25-Arg617

Attributs du produit: Recombinant Rat Coagulation Factor II Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Arg617.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Autre désignation: Coagulation Factor II (F2 Produits)

Synonymes: PT Protein, RPRGL2 Protein, THPH1 Protein, Cf-2 Protein, Cf2 Protein, FII Protein, thrombin Protein, zgc:66319 Protein, wu:fb57c10 Protein, wu:fd42e09 Protein, wu:fd59d01 Protein, prothrombin Protein, F2 Protein, coagulation factor II, thrombin Protein, coagulation factor II Protein, coagulation factor II (thrombin) Protein, F2 Protein, f2 Protein

Sujet: Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites.

Poids moléculaire: 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_075213

Pathways: Système du Complément, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling