TNF alpha Protéine

N° du produit ABIN7539350

Cette protéine Recombinant TNF alpha est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TNF alpha Protéine (ABIN7539350)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE and RP-HPLC analysis

Détail du produit

Fonction: TNF-alpha

Séquence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Specificité: Chromosomal location:6p21.3

Attributs du produit: Length (aa):158

Information d'application

Indications d'application: The ED50 as determined by the proliferation assay with TF-1 cells is in the range of 0.05-0.4 ng/mL.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized TNF-alpha is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in sterile water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: 20 mM PB, ph 7.2 + 100 mM NaCl

Stock: RT,4 °C,-20 °C

Stockage commentaire: Lyophilized Tumor Necrosis Factor-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha

Sujet: TNF, DIF, TNFA, TNFSF2, TNF-alpha,Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. Human recombinant Tumor Necrosis Factor-α produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Dalton.

Poids moléculaire: 17.5 kDa

ID gène: 7124

NCBI Accession: NM_000594, NP_000585

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome