TNF alpha Protein (AA 80-235)

N° du produit ABIN7607268

Protéine Recombinant TNF alpha exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TNF alpha Protein (AA 80-235) (ABIN7607268)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Immunogen (Imm), Activity Assay (AcA)

Pureté: >90 % as determined by SDS-PAGE.

Détail du produit

Attributs du protein: AA 80-235

Fonction: Recombinant Mouse TNFa/TNF-alpha Protein, No tag (Active)

niveau d'endotoxine: <1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Recombinant Mouse TNFa/TNF-alpha (Cat #: AMB94401) induces cytotoxicity in the mouse fibroblast cells (L-929) in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 29-116 pg/mL, corresponding to a specific activity of 8.62x10^6-3.45x10^7 units/mg.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Date de péremption: 12 months

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor necrosis factor

Sujet: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome