anti-Humain BAD anticorps pour Immunohistochemistry (Paraffin-embedded Sections)

Recommended BAD Antibody (fourni par: Connectez-vous pour afficher )

Antigène
BCL2-Associated Agonist of Cell Death (BAD) Anticorps
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Épitope
AA 140-168
58
57
52
48
47
45
42
39
29
22
22
19
19
19
19
19
19
19
19
19
19
19
19
19
19
18
18
18
18
18
18
15
15
12
10
9
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
760
441
274
19
15
8
5
4
3
2
2
1
1
1
1
1
1
Hôte
Lapin
970
48
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp BAD est non-conjugé
67
66
66
60
60
60
6
6
6
6
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
526
503
396
294
122
78
52
30
13
12
6
5
4
3
2
1
1
1
Options
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N° du produit (Fournisseur)
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N° du produit ABIN674709
$ 306.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
12.824656 ABIN461986 IHC (p) IP WB Mouse IgG N-Term Connectez-vous pour afficher BYC001 0
12.824656 ABIN4282857 IHC IHC (p) WB Rabbit Connectez-vous pour afficher Polyclonal 1
12.824656 ABIN213744 IHC (p) WB Rabbit IgG pSer112 Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN214418 IHC (p) WB Rabbit C-Term Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN498774 IHC (p) WB Rabbit pSer112 Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN498190 IF IHC (p) WB Rabbit Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN498864 IHC (p) Rabbit Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN682828 IF (p) IHC (p) WB Rabbit IgG pSer75 Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN756562 IF (p) IHC (p) WB Rabbit IgG pSer118 Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN4282865 IHC (p) IP WB Mouse IgG N-Term Connectez-vous pour afficher BYC001 0
12.824656 ABIN498091 IHC (p) WB Rabbit Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN4282859 ICC IF IHC IHC (p) IP WB Rabbit Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN5557534 IHC (p) WB Rabbit IgG Connectez-vous pour afficher 5D4 0
12.824656 ABIN4282868 ICC IF IHC IHC (p) WB Rabbit IgG Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN4282853 ICC IF IHC IHC (p) WB Rabbit pSer155 Connectez-vous pour afficher Polyclonal 0
12.824656 ABIN4282864 IHC IHC (p) IP WB Rabbit Connectez-vous pour afficher Polyclonal 0
1 ABIN358087 EIA IHC (p) WB Rabbit Ig Fraction pSer99 Connectez-vous pour afficher Polyclonal 5
1 ABIN389521 DB IF IHC (p) Rabbit Ig Fraction pSer99 Connectez-vous pour afficher Polyclonal 2
1 ABIN389519 IHC (p) WB Rabbit Ig Fraction pSer134 Connectez-vous pour afficher Polyclonal 2
1 ABIN388118 FACS IHC (p) WB Rabbit Ig Fraction AA 92-127, BH3 Domain Connectez-vous pour afficher Polyclonal 3

General

Antigène BCL2-Associated Agonist of Cell Death (BAD) Anticorps
Épitope AA 140-168
(58), (57), (52), (48), (47), (45), (42), (39), (29), (22), (22), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (18), (18), (18), (18), (18), (15), (15), (12), (10), (9), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris
(760), (441), (274), (19), (15), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Hôte Lapin
(970), (48), (2), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp BAD est non-conjugé
(67), (66), (66), (60), (60), (60), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(526), (503), (396), (294), (122), (78), (52), (30), (13), (12), (6), (5), (4), (3), (2), (1), (1), (1)
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Détail du produit anti-BAD anticorps

Détail du antigène BAD Information d'application Stockage Images
Purification Purified by Protein A.
Immunogène KLH conjugated synthetic peptide derived from human Bad
Isotype IgG

Détail du antigène BAD

Détail du produit anti-BAD anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation Bad (BAD Antibody Extrait)
Sujet

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Subcellular location: Cytoplasm, Mitochondrion

Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6

ID gène 572
UniProt Q92934
Pathways Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Information d'application

Détail du produit anti-BAD anticorps Détail du antigène BAD Stockage Images Haut de la page
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Stockage

Détail du produit anti-BAD anticorps Détail du antigène BAD Information d'application Images Haut de la page
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months

Images

Détail du produit anti-BAD anticorps Détail du antigène BAD Information d'application Stockage Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal A...
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Image provided by the Independent Validation Program (badge number 29802). Lane 1: MC...
'Independent Validation' signe
Numéro du lot 090915
Application validée Western Blotting
Contrôle positif MCF7 cells
Contrôle négative C6/36 cells (non-reactive species)
Conclusion A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
Anticorps primaire
Anticorps secondaire
Contrôles
  • Positive control: MCF7 cells
  • Negative control: C6/36 cells
Protocole
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Figure 1: Western Blot for BAD. Arrowhead indicates the expected molecular weight of ...