anti-Souris KIT anticorps pour Immunoprecipitation

Recommended KIT Antibody

Antigène
Mast/stem Cell Growth Factor Receptor (KIT) Anticorps
  • C-Kit
  • CD117
  • PBT
  • SCFR
  • Bs
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
  • c-KIT
  • c-kit
  • MGF
  • KIT
  • C-KIT
  • kit
  • receptor
  • sparse
  • Xkl-1
  • kit-a
  • kit-b
  • kl1-A
  • krk1
  • pbt
  • scfr
  • KIT proto-oncogene receptor tyrosine kinase
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a
  • KIT proto-oncogene receptor tyrosine kinase S homeolog
  • KIT
  • Kit
  • kita
  • kit
  • kit.S
Reactivité
Souris
1325
468
170
45
44
38
26
19
14
5
4
4
4
3
2
1
1
1
Hôte
Rat
1055
360
161
14
2
2
Clonalité
Monoclonal
Conjugué
Cet anticorp KIT est non-conjugé
146
134
128
109
86
76
21
17
15
14
14
14
8
8
8
8
8
8
6
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
675
628
442
347
214
151
117
105
71
68
55
29
19
13
11
10
3
2
2
2
1
1
1
Options
N° du produit ABIN2688894
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13.656681 ABIN2135863 Func FACS IP IHC Rat IgG2b kappa 2B8 0
10 ABIN2895728 IF/ICC IHC IP WB Rabbit AA 343-527 Polyclonal 0
7 ABIN2660261 FACS IP ELISA PerCP-Cy5.5 Rat IgG2b kappa 2B8 7
6.3423786 ABIN5550755 FACS ICC IF IHC IHC (fro) IP Rat IgG2b kappa 2B8 10
4 ABIN2662960 FACS IF IP WB PE Rat IgG2b kappa ACK2 6
4 ABIN2664146 BR FACS IP ELISA WB Rat IgG2b kappa ACK2 6
4 ABIN6923556 ELISA IP WB Rabbit IgG AA 1-523 017 0
1 ABIN2660262 FACS IP ELISA PerCP-Cy5.5 Rat IgG2b kappa 2B8 7
1 ABIN2662961 FACS IF IP WB PE Rat IgG2b kappa ACK2 6
1 ABIN2664145 BR FACS IP ELISA WB Rat IgG2b kappa ACK2 6
1 ABIN476595 FACS IHC IHC (fro) IP Rat IgG2b, kappa ACK2 0
1 ABIN474861 FACS IP Rat IgG2b, kappa 2B8 0
1 ABIN2222190 FACS IP IHC Rat IgG2b kappa 2B8 0
1 ABIN2222176 FACS IP IHC WB Rat IgG2b kappa 2Q195 0
1 ABIN6723034 FACS IF IHC IP Rat IgG2b, kappa 2B8 0
1 ABIN6725879 FACS IF IHC IP Rat IgG2b, kappa 2B8 0
1 ABIN1721425 IHC (fro) FACS IP DyLight 650 Rat IgG2b 2B8 0
1 ABIN1721426 IHC (fro) FACS IP DyLight 488 Rat IgG2b 2B8 0
1 ABIN1721427 IHC (fro) FACS IP DyLight 550 Rat IgG2b 2B8 0
1 ABIN6345161 IF/ICC IHC IP WB Rabbit Polyclonal 0

General

Antigène Mast/stem Cell Growth Factor Receptor (KIT) Anticorps
Reactivité Souris
(1325), (468), (170), (45), (44), (38), (26), (19), (14), (5), (4), (4), (4), (3), (2), (1), (1), (1)
Hôte Rat
(1055), (360), (161), (14), (2), (2)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp KIT est non-conjugé
(146), (134), (128), (109), (86), (76), (21), (17), (15), (14), (14), (14), (8), (8), (8), (8), (8), (8), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(675), (628), (442), (347), (214), (151), (117), (105), (71), (68), (55), (29), (19), (13), (11), (10), (3), (2), (2), (2), (1), (1), (1)
Pubmed 15 références disponible
Fournisseur

Détail du produit anti-KIT anticorps

Détail du antigène KIT Information d'application Stockage References for anti-KIT antibody (ABIN2688894) Images
Marque BD Pharmingen™
Attributs du produit The 2B8 antibody reacts with CD117 (c-Kit), a transmembrane tyrosine-kinase receptor which is encoded by the Kit gene (formerly dominant white spotting, W). The c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor) encoded by the Kit1 gene (formerly steel, SI), is a co-mitogen for hematopoietic stem cells, myeloerythroid progenitors and a mast-cell differentiation factor. The KitW and Kit1SI mutant alleles have similar pleiotropic effects on the development of melanocytes, germ cells, and the hematopoietic system. In the adult bone marrow, CD117 is expressed on hematopoietic progenitor cells, including CD90 (Thy-1) low, TER-119-, CD45R/B220-, CD11b (Mac-1)-, Ly-6G (Gr-1)-, CD4-, CD8-, and Sca-1 (Ly-6A/E)+ multipotent hemotopoietic stem cells, progenitors committed to myeliod and/or erythroid lineages, and precursors of B and T lymphocytes. This widespread expression of CD117 in hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages. Intrathymic expression of c-Kit and c-Kit ligand suggest that CD117 is also involved in the regulation of some events during the development of T lymphocytes. CD117 is also expressed by mast cells and by dendritic cells found in the periarteriolar lymphocytoc sheaths (T-cell areas) of splenic white pulp. The mAb 2B8 reportedly does not block the action of c-Kit. This clone 2B8 had been reported that there was cross-reactivity with rat. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two color analysis of the expression of CD117 on mouse bone marrow cells. A single-cell suspension of BALB/c bone marrow was simultaneously stained with PE-conjugated RA3-6B2 (anti-mouse CD45R/B220, Cat. No. 553089/553090, both panels) and purified 2B8 (bottom panel) monoclonal antibodies, followed by FITC-conjugated anti-rat IgG2b mAb RG7/11.1 (Cat. No. 553900, both panels). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Rat Anti-Mouse CD117 - Purified - Clone 2B8 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Mouse Bone Marrow Mast Cells
Clone 2B8
Isotype IgG2b, kappa
Plasmids, Primers & others

Détail du antigène KIT

Détail du produit anti-KIT anticorps Information d'application Stockage References for anti-KIT antibody (ABIN2688894) Images Haut de la page
Antigène
Autre désignation CD117 (KIT Antibody Extrait)
Sujet Synonyms: c-Kit
Pathways Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity

Information d'application

Détail du produit anti-KIT anticorps Détail du antigène KIT Stockage References for anti-KIT antibody (ABIN2688894) Images Haut de la page
Indications d'application Flow Cytometry System.
Restrictions For Research Use only

Stockage

Détail du produit anti-KIT anticorps Détail du antigène KIT Information d'application References for anti-KIT antibody (ABIN2688894) Images Haut de la page
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store undiluted at 4°C.

References for anti-KIT antibody (ABIN2688894)

Détail du produit anti-KIT anticorps Détail du antigène KIT Information d'application Stockage Images Haut de la page
Produit citée dans:

Fadini, Sartore, Schiavon, Albiero, Baesso, Cabrelle, Agostini, Avogaro: "Diabetes impairs progenitor cell mobilisation after hindlimb ischaemia-reperfusion injury in rats." dans: Diabetologia, Vol. 49, Issue 12, pp. 3075-84, 2006

Austen, Boyce: "Mast cell lineage development and phenotypic regulation." dans: Leukemia research, Vol. 25, Issue 7, pp. 511-8, 2001

Domen, Weissman: "Hematopoietic stem cells need two signals to prevent apoptosis; BCL-2 can provide one of these, Kitl/c-Kit signaling the other." dans: The Journal of experimental medicine, Vol. 192, Issue 12, pp. 1707-18, 2001

Mirmonsef, Shelburne, Fitzhugh Yeatman, Chong, Ryan: "Inhibition of Kit expression by IL-4 and IL-10 in murine mast cells: role of STAT6 and phosphatidylinositol 3'-kinase." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2530-9, 1999

Lian, Toki, Yu, Hayashi, Yasumizu, Sugiura, Jin, Inaba, Hisha, Li, Yu, Fan, Ikehara: "Intrathymically injected hemopoietic stem cells can differentiate into all lineage cells in the thymus: differences between c-kit+ cells and c-kit < low cells." dans: Stem cells (Dayton, Ohio), Vol. 15, Issue 6, pp. 430-6, 1998

Kondo, Weissman, Akashi: "Identification of clonogenic common lymphoid progenitors in mouse bone marrow." dans: Cell, Vol. 91, Issue 5, pp. 661-72, 1997

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, 1997

Rodewald, Kretzschmar, Swat, Takeda: "Intrathymically expressed c-kit ligand (stem cell factor) is a major factor driving expansion of very immature thymocytes in vivo." dans: Immunity, Vol. 3, Issue 3, pp. 313-9, 1995

Godfrey, Zlotnik: "Control points in early T-cell development." dans: Immunology today, Vol. 14, Issue 11, pp. 547-53, 1994

Valent: "The riddle of the mast cell: kit(CD117)-ligand as the missing link?" dans: Immunology today, Vol. 15, Issue 3, pp. 111-4, 1994

Ikuta, Weissman: "Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1502-6, 1992

Ogawa, Matsuzaki, Nishikawa, Hayashi, Kunisada, Sudo, Kina, Nakauchi, Nishikawa: "Expression and function of c-kit in hemopoietic progenitor cells." dans: The Journal of experimental medicine, Vol. 174, Issue 1, pp. 63-71, 1991

Anderson, Lyman, Baird, Wignall, Eisenman, Rauch, March, Boswell, Gimpel, Cosman: "Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms." dans: Cell, Vol. 63, Issue 1, pp. 235-43, 1990

Huang, Nocka, Beier, Chu, Buck, Lahm, Wellner, Leder, Besmer: "The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus." dans: Cell, Vol. 63, Issue 1, pp. 225-33, 1990

Zsebo, Wypych, McNiece, Lu, Smith, Karkare, Sachdev, Yuschenkoff, Birkett, Williams: "Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium." dans: Cell, Vol. 63, Issue 1, pp. 195-201, 1990