NF-kB p65 anticorps (C-Term)

N° du produit ABIN116521

Cet anticorps Lapin Polyclonal détecte spécifiquement NF-kB p65 dans WB, IP, EIA et EMSA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NF-kB p65 anticorps (C-Term) (ABIN116521)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Détail du produit anti-NF-kB p65 anticorps

Épitope: C-Term

Specificité: This antibody may react non-specifically with other proteins. Control Peptide (#R1009CP) will compete only with the specific reaction of antiserum with NFkB p65 (Rel A) subunit.

Purification: Prepared from monospecific antiserum by Delipidation and Defibrination.

Immunogène: Human NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH)

Information d'application

Indications d'application: Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against NFkB p65 (Rel A) at a dilution of 1/2000 to 1/5000 followed by reactionwith Peroxidase conjugated Goat anti-Rabbit IgG (H&L) (Cat#R1364HRP). Anti-NFkB p65 (RelA) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A). This product was also tested in a gel supershift assay and found to be reactive against allp65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 μL per assay. Gel (Super) Shift Information: NFkB gel shift assays are assembled in 20 μL reactions containing 0.28 pmoles NFkB oligoin 10 mM Tris ( pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10 % glycerol. Someprocedures specify the addition of 0.05 % NP-40. When using purified protein, 250-300 ngshould be sufficient to produce a gel shifted complex, while 10 μg HeLa nuclear extract isutilized. The gel shift reactions are then incubated at room temperature for 30 minutes. The

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 80.0 mg/mL (by Refractometry)

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: RELA / NF-kB p65

Sujet: NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-alpha. IkB-alpha binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65

ID gène: 5970

NCBI Accession: NP_001138610

UniProt: Q04206

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins