Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Séquence
M-T-S-A-L-A-T-M-R-V-D-Y-E-Q-I-K
Purification
Affinity Purified
Immunogène
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
MAPKAPK2
Reactivité: Humain
IHC (p), DB
Hôte: Lapin
Polyclonal
RB05555
unconjugated
Indications d'application
Suitable for use in Western blotting, ELISA, Immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. Affinity purified anti-MAPKAP Kinase 2 is specific for MAPKAPKinase 2 and will not effectively immunoblot other isoforms of the kinase (i. e. MAPKAP K1or K3) from cell lines and/or tissues. Use a HeLa cell lysate as a positive control. MK2assays are often used and are easier and have less background than p38 kinase assays. Immunoprecipitation of MK2 using antibodies is used for this purpose. This product wasassayed by immunoblot and was found to be reactive to 20 g of crude protein extractprepared from HeLa cells. The gel was probed with Affinity Purified anti-MAPKAP Kinase2used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG(H&L) (GOAT) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) as a secondary
Restrictions
For Research Use only
Concentration
0.9 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 2-8 °C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." dans: Transplantation, Vol. 85, Issue 3, pp. 398-406, (2008) (PubMed).
Antigène
MAPKAP Kinase 2 (MAPKAPK2)
(Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
anticorps mapkapk2, anticorps wu:fb30e10, anticorps wu:fc56c10, anticorps wu:fi03a01, anticorps MGC78852, anticorps MAPKAPK2, anticorps AA960234, anticorps MAPKAP-K2, anticorps MK-2, anticorps MK2, anticorps Rps6kc1, anticorps mitogen-activated protein kinase-activated protein kinase 2a, anticorps mitogen-activated protein kinase-activated protein kinase 2 L homeolog, anticorps mitogen-activated protein kinase-activated protein kinase 2, anticorps MAP kinase-activated protein kinase 2, anticorps mapkapk2a, anticorps mapkapk2.L, anticorps MAPKAPK2, anticorps Mapkapk2
Sujet
MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-α at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2