MAPK14 anticorps (pThr180)
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- Antigène Voir toutes MAPK14 Anticorps
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
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Épitope
- pThr180
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Reactivité
- Humain, Souris, Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MAPK14 est non-conjugé
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Application
- Western Blotting (WB), Intracellular Staining (ICS), BioImaging (BI)
- Réactivité croisée
- Souris, Rat (Rattus)
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Phosphorylated Human p38 MAPK (pT180/pY182) Peptide
- Clone
- 36-p38
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MAPK14 Anticorps primaire
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- Indications d'application
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Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 min. at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate 1 hr.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hr.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. - Commentaires
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Related Products: ABIN968535, ABIN968632, ABIN968769
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store undiluted at -20°C.
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Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, (1997) (PubMed).
: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." dans: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, (1996) (PubMed).
: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." dans: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, (1994) (PubMed).
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Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, (1997) (PubMed).
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- Antigène
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
- Autre désignation
- p38 MAPK (MAPK14 Produits)
- Synonymes
- anticorps CSBP, anticorps CSBP1, anticorps CSBP2, anticorps CSPB1, anticorps EXIP, anticorps Mxi2, anticorps PRKM14, anticorps PRKM15, anticorps RK, anticorps SAPK2A, anticorps p38, anticorps p38ALPHA, anticorps CRK1, anticorps Csbp1, anticorps Csbp2, anticorps Exip, anticorps Hog, anticorps Prkm14, anticorps Prkm15, anticorps Sapk2A, anticorps p38Hog, anticorps p38alpha, anticorps p38b, anticorps zp38b, anticorps MAPK14, anticorps 186F5S, anticorps BG:DS00797.3, anticorps CG7393, anticorps D-p38, anticorps D-p38 MAPK, anticorps D-p38b, anticorps Dm p38b, anticorps Dmel\\CG7393, anticorps Dmp38b, anticorps Dp38, anticorps Dp38b, anticorps ESTS:186F5S, anticorps Mpk34C, anticorps anon-sts23, anticorps dp38b, anticorps p38 MAPK, anticorps p38 beta, anticorps p38B, anticorps p38Kb, anticorps p38beta, anticorps Crk1, anticorps p38-alpha, anticorps p38MAPK, anticorps p38a, anticorps csbp, anticorps mapk14a, anticorps mxi2, anticorps sapk2, anticorps sapk2a, anticorps AP22.98, anticorps AP22_98, anticorps ATMPK14, anticorps mitogen-activated protein kinase 14, anticorps SAPK2a, anticorps MAP kinase 14A, anticorps MAP kinase p38a, anticorps MAPK 14A, anticorps fk28c03, anticorps hm:zeh1243, anticorps wu:fk28c03, anticorps zp38a, anticorps P38C-CRK, anticorps mitogen-activated protein kinase 14, anticorps mitogen activated protein kinase 14, anticorps mitogen-activated protein kinase 14b, anticorps p38b MAP kinase, anticorps mitogen-activated protein kinase 14 S homeolog, anticorps mitogen-activated protein kinase 14a, anticorps CRK proto-oncogene, adaptor protein, anticorps MAPK14, anticorps Mapk14, anticorps mapk14b, anticorps p38b, anticorps mapk14.S, anticorps MPK14, anticorps mapk14a, anticorps CRK
- Sujet
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Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the Mitogen Activated Protein Kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38alpha (MAPK14), beta (MAPK11), gamma (MAPK12), and delta (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP Kinase Kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways.
The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38alpha, beta, gamma, and delta.
Synonyms: MK14, 11, 12, 13, CSBP1, SAPK2, 2A, 3, 4, MX12, ERK-6, ERK5 - Poids moléculaire
- 38-42 kDa
- Pathways
- Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
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