KCNMA1 anticorps (Internal Region)
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- Antigène Voir toutes KCNMA1 Anticorps
- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
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Épitope
- Internal Region
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Reactivité
- Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Cheval, Xenopus laevis, Poulet, Singe, Porc, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp KCNMA1 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human KCNMA1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 15 amino acid peptide from internal region of human KCNMA1. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product KCNMA1 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (10 - 20 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
- Autre désignation
- KCNMA1 / BK (KCNMA1 Produits)
- Synonymes
- anticorps BKTM, anticorps KCa1.1, anticorps MaxiK, anticorps SAKCA, anticorps SLO, anticorps SLO-ALPHA, anticorps SLO1, anticorps bA205K10.1, anticorps mSLO1, anticorps DDBDRAFT_0190653, anticorps DDBDRAFT_0235401, anticorps DDB_0190653, anticorps DDB_0235401, anticorps KCNMA, anticorps KCNMA1, anticorps 5730414M22Rik, anticorps BKCa, anticorps Slo, anticorps Slo1, anticorps mSlo, anticorps mSlo1, anticorps KCNMA1b, anticorps KCNMA1c, anticorps Kcnma, anticorps bktm, anticorps kcnma1-A, anticorps sakca, anticorps cSlo, anticorps slo1, anticorps kcnma1, anticorps si:ch211-39f22.2, anticorps BcDNA:GH10751, anticorps CG10693, anticorps Dmel\CG10693, anticorps Dslo, anticorps dSlo, anticorps dSlo1, anticorps dslo, anticorps fSlo, anticorps potassium calcium-activated channel subfamily M alpha 1, anticorps calcium-activated BK potassium channel, alpha subunit, anticorps potassium large conductance calcium-activated channel, subfamily M, alpha member 1, anticorps potassium calcium-activated channel subfamily M alpha 1 L homeolog, anticorps potassium large conductance calcium-activated channel, subfamily M, alpha member 1a, anticorps calcium-activated potassium channel subunit alpha-1, anticorps slowpoke, anticorps KCNMA1, anticorps kcnma1, anticorps Kcnma1, anticorps kcnma1.L, anticorps kcnma1a, anticorps LOC100454095, anticorps slo
- Sujet
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Name/Gene ID: KCNMA1
Subfamily: Potassium channel - calcium-activated
Family: Ion Channel
Synonyms: KCNMA1, BA205K10.1, BKCA alpha subunit, BK channel, BKCA alpha, BKTM, HSlo, K(VCA)alpha, KCa1.1, Maxi-K channel HSLO, MaxiK, KCNMA, SAKCA, SLO-ALPHA, Slowpoke homolog, Stretch-activated Kca channel, Slo homolog, BK channel alpha subunit, Maxi K channel, MSlo, MSLO1, SLO, SLO1 - ID gène
- 3778
- Pathways
- Regulation of Hormone Metabolic Process, Sensory Perception of Sound
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