TRKB anticorps (Cytoplasmic Domain)

N° du produit ABIN1049153

Détail du produit anti-TRKB anticorps

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Épitope: Cytoplasmic Domain

Reactivité: Humain, Singe, Lapin, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRKB est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human NTRK2 / TRKB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%) Bovine (92%) Galago, Bat (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 12 amino acid peptide from cytoplasmic domain of human NTRK2 / TRKB. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%), Bovine (92%), Galago, Bat (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (20 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur TRKB

Antigène: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Autre désignation: NTRK2 / TRKB (NTRK2 Produits)

Synonymes: anticorps trkb, anticorps trkb-b, anticorps xtrkb, anticorps si:ch211-220b11.1, anticorps trkB2, anticorps NTRK2, anticorps ntrk2, anticorps gp145-trkb, anticorps TRKB, anticorps GP145-TrkB, anticorps trk-B, anticorps AI848316, anticorps C030027L06Rik, anticorps Tkrb, anticorps trkB, anticorps RATTRKB1, anticorps TRKB1, anticorps Trk-B, anticorps TrkB, anticorps neurotrophic receptor tyrosine kinase 2, anticorps neurotrophic receptor tyrosine kinase 2 L homeolog, anticorps neurotrophic tyrosine kinase, receptor, type 2b, anticorps neurotrophic receptor tyrosine kinase 2 S homeolog, anticorps neurotrophic tyrosine kinase, receptor, type 2, anticorps NTRK2, anticorps ntrk2.L, anticorps ntrk2b, anticorps ntrk2.S, anticorps ntrk2, anticorps Ntrk2

Sujet: Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase

Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B

ID gène: 4915

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport