CD4 anticorps
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- Antigène Voir toutes CD4 Anticorps
- CD4
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Reactivité
- Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD4 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- Purification
- Affinity chromatography.
- Immunogène
- Lymphoblasts.
- Clone
- Rib 5-2
- Isotype
- IgG2a
- Top Product
- Discover our top product CD4 Anticorps primaire
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- Indications d'application
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Suitable for Immunohistochemistry (IHC), has been described to work in FACS (see Table1). Recommended Dilutions for IHC: Frozen sections: 1.5 μg/mL (1/200)Paraffin sections: does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water.
- Concentration
- 0.3 mg/mL
- Buffer
- PBS, pH 7.2 containing 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- Antigène
- CD4
- Autre désignation
- CD4 (CD4 Produits)
- Synonymes
- anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
- Sujet
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- ID gène
- 10116
- UniProt
- P05540
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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