VEGFA anticorps (N-Term)

N° du produit ABIN115674

Détail du produit anti-VEGFA anticorps

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Épitope: N-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGFA est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: The antibody will react with all VEGF-A isoforms.

Purification: Affinity Chromatography with immobilized Protein A

Immunogène: Highly purified N-terminal 20 amino acid peptide of Human VEGF-A

Information d'application

Indications d'application: ELISA: To detect Human VEGF by Direct ELISA a concentration of at least 0.5-2 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 1,0- 2,5 ng/well of recombinant Human VEGF165 or other VEGF splice forms. Western Blot: 5-10 μg/mL. It will detect 25 ng/lane of recombinant human and murineVEGF-A under reducing conditions, dimers are detected at higher protein concentrations.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/mL

Buffer: PBS, pH 7.4 containing no preservative

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized IgG is stable at 2-8 °C for at least one month and for longer when kept at -20 °C. When reconstituted the antibody is stable for at least six weeks at 2-4 °C.

Détails sur VEGFA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A (VEGFA Produits)

Synonymes: anticorps MVCD1, anticorps VEGF, anticorps VPF, anticorps Vegf, anticorps Vegf120, anticorps Vegf164, anticorps Vegf188, anticorps Vpf, anticorps vegf, anticorps vegfa, anticorps wu:fj82c06, anticorps VEGF-A, anticorps VEGF164, anticorps eVEGF120, anticorps eVEGF164, anticorps vegf-a, anticorps vpf, anticorps vefg, anticorps si:dkey-14d8.3, anticorps wu:fd42e02, anticorps vascular endothelial growth factor A, anticorps vascular endothelial growth factor Aa, anticorps vascular endothelial growth factor A L homeolog, anticorps vascular endothelial growth factor Ab, anticorps VEGFA, anticorps Vegfa, anticorps vegfaa, anticorps vegfa.L, anticorps vegfa, anticorps vegfab

Sujet: VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

ID gène: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling