Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-10 (1.5 ng/mL), a concentration of 0.02-0.035 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFGF-10 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFGF-10. (Sandwich): To detect hFGF-10 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human FGF-10 (PP1119B1 or PP1119B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFGF-10. Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry on frozen or paraffin sections: This antibody should be used at0.5-2.0 μg/mL depending on dection system. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
FGF10 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs.Synonyms: FGF-10, Fibroblast growth factor 10, KGF2, Keratinocyte growth factor 2