IGF1 anticorps (Biotin)
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- Antigène Voir toutes IGF1 Anticorps
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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Reactivité
- Souris
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IGF1 est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- Murine IGF-1
- Purification
- Affinity chromatography.
- Immunogène
- Highly pure (>98%) recombinant mIGF-1.
- Top Product
- Discover our top product IGF1 Anticorps primaire
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- Indications d'application
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ELISA(Direct): To detect mIGF-I by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIGF-I. (Sandwich): To detect mIGF-I by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine IGF-I (PP1120P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant mIGF-I. Western Blot: To detect mIGF-I by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIGF-I is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Antigène
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- Autre désignation
- Insulin-Like Growth Factor I / IGF1 (IGF1 Produits)
- Synonymes
- anticorps IGF-I, anticorps IGF1A, anticorps IGFI, anticorps C730016P09Rik, anticorps Igf-1, anticorps Igf-I, anticorps Npt2B, anticorps IGF-1, anticorps IGFIA, anticorps IGF-IB, anticorps igf1, anticorps IGF-1L, anticorps IGF-1a, anticorps IGF1, anticorps igf-1, anticorps igf1-A, anticorps igf1.S, anticorps xigf1, anticorps insulin like growth factor 1, anticorps insulin-like growth factor 1, anticorps insulin-like growth factor I, anticorps insulin like growth factor 1 L homeolog, anticorps IGF1, anticorps Igf1, anticorps LOC100136741, anticorps igf1, anticorps LOC678666, anticorps igf1.L, anticorps LOC104911603
- Sujet
- Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.Synonyms: IBP1, IGF-I, Somatomedin
- ID gène
- 16000
- NCBI Accession
- NP_001104745
- UniProt
- P05017
- Pathways
- Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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