CD137 anticorps (Biotin)

N° du produit ABIN116396

Détail du produit anti-CD137 anticorps (Biotin)

Antigène: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CD137 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts with 4-1BB Receptor.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant h4-1BB Receptor.

Information d'application

Indications d'application: ElISA: (Direct: To detect h4-1BB Receptor by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant h4-1BB Receptor. (Sandwich): To detect h4-1BB Receptor by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human 4-1BB Receptor PP1201P asa capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant h4-1BBReceptor. Western Blot: To detect h4-1BB Receptor by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant h4-1BB Receptor is 1.5-3.0 ng/lane, under

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD137

Antigène: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Autre désignation: CD137 / TNFRSF9 (TNFRSF9 Produits)

Synonymes: anticorps TNFRSF9, anticorps zgc:136557, anticorps 4-1BB, anticorps CD137, anticorps CDw137, anticorps ILA, anticorps A930040I11Rik, anticorps AA408498, anticorps AI325004, anticorps Cd137, anticorps Ly63, anticorps tumor necrosis factor receptor superfamily member 9, anticorps TNF receptor superfamily member 9, anticorps tumor necrosis factor receptor superfamily, member 9a, anticorps tumor necrosis factor receptor superfamily, member 9, anticorps TNFRSF9, anticorps tnfrsf9a, anticorps Tnfrsf9

Sujet: CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60 % amino acid identity. CD137 (4-1BB), a member of the tumour necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival.Synonyms: 4-1BB homolog, 4-1BB ligand receptor, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9

ID gène: 3604

NCBI Accession: NP_001552

UniProt: Q07011

Pathways: Cancer Immune Checkpoints