CIITA anticorps (C-Term)

N° du produit ABIN116528

Détail du produit anti-CIITA anticorps

Antigène: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Épitope: AA 726-742, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CIITA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Séquence: CGEIKDNKEL PQYLALTTR

Specificité: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Cross reactivity against CIITA from other sources may occur but has not been specifically determined.

Purification: Multi-step process.

Immunogène: Peptide corresponding to a region near the C-terminal end (amino acids 726-742) of the human CIITA gene conjugated to Keyhole Limpet Hemocyanin (KLH).

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed by immunoblot against CIITAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 is suggested for this product. Best Results are obtained using 0.03 % (v/v) Triton X-100 IN BUFFERS. Do not supplement buffers with Tween-20 detergent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store original vial and reconstituted antibody at -20 °C.

Détails sur CIITA

Antigène: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Autre désignation: CIITA / C2TA (CIITA Produits)

Synonymes: anticorps CIITA, anticorps C2ta, anticorps EG669998, anticorps Gm9475, anticorps C2TA, anticorps CIITAIV, anticorps MHC2TA, anticorps NLRA, anticorps Mhc2ta, anticorps class II, major histocompatibility complex, transactivator, anticorps class II major histocompatibility complex transactivator, anticorps MHC class II transactivator, anticorps class II transactivator, anticorps CIITA, anticorps LOC710761, anticorps ciita, anticorps LOC100443575, anticorps Ciita, anticorps LOC100736732

Sujet: The transactivator CIITA regulates basal and interferon-induced expression of MHC class II genes. CIITA restores expression of all MHC II gene expression in mutant cells and corrects regulatory defects of those genes. Activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. CIITA may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter.Synonyms: MHC class II transactivator, MHC2TA

ID gène: 4261

NCBI Accession: NP_000237

UniProt: P33076

Pathways: Cancer Immune Checkpoints