beta-2 Microglobulin anticorps (HRP)

N° du produit ABIN116647

Détail du produit anti-beta-2 Microglobulin anticorps (HRP)

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta-2 Microglobulin est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Specificité: This product is an IgG fraction antibody purified from monospecific antiserum by a multi- step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified beta-2-Microglobulin [Human Urine]. Cross reactivity against beta-2-Microglobulin from other sources is unknown.

Purification: Multistep Process.

Immunogène: Beta-2-Microglobulin [Human Urine].

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Stock: 4 °C

Stockage commentaire: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.

Date de péremption: 12 months

Détails sur beta-2 Microglobulin

Antigène: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Autre désignation: Beta-2-microglobulin (B2M Produits)

Synonymes: anticorps Ly-m11, anticorps beta2-m, anticorps beta2m, anticorps bwm, anticorps wu:fa94c05, anticorps wu:fb10a09, anticorps b2m-W01, anticorps b2m-W03, anticorps b2m-Z01, anticorps b2m-Z02, anticorps b2m-Z03, anticorps B2M, anticorps b2m, anticorps B2MG, anticorps wu:fb66f11, anticorps wu:fj01f08, anticorps zgc:64161, anticorps b-2-m, anticorps beta-2-microglobulin, anticorps beta-2 microglobulin, anticorps beta-2-microglobulin S homeolog, anticorps beta-2-microglobulin, like, anticorps B2M, anticorps B2m, anticorps b2m, anticorps b2m.S, anticorps b2ml

Sujet: Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Alternate names: B2M, Beta 2 microglobulin, Beta-2 microglobulin

ID gène: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process