NF-kB p65 anticorps (pSer276)

N° du produit ABIN116928

Cet anticorps anti-NF-kB p65 Polyclonal Lapin (ABIN116928) détecte spécifiquement NF-kB p65 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour NF-kB p65 anticorps (pSer276) (ABIN116928)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NF-kB p65 anticorps

Épitope: pSer276

Séquence: QLRRPpSDRE LSC

Specificité: This phospho specific polyclonal antibody is specific for phosphorylated pS276 p65. Reactivity with non-phosphorylated p65 is minimal.

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogène: NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Information d'application

Indications d'application: This phospho specific polyclonal antibody reacts human pS276 p65 and shows minimalreactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISAagainst the non-phosphorylated form of the immunizing peptide. For immunoblotting a 1: 1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5cells can be used as a positive control. For ELISA a 1: 10,000 to 1: 30,000 dilution is recommended. Although not tested, this antibody is likely functional in immunohistochemistry andimmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 80.0 mg/mL (by refractometry)

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: RELA / NF-kB p65

Sujet: NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators. NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65. NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65

ID gène: 5970

NCBI Accession: NP_001138610

UniProt: Q04206

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins