NFKBIA anticorps (AA 145-302) (PE)

N° du produit ABIN1176898

Détail du produit anti-NFKBIA anticorps (PE)

Antigène: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Épitope: AA 145-302

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NFKBIA est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human IkappaBalpha aa. 145-302 Recombinant Protein

Clone: 25-IkBa-MAD

Isotype: IgG1

Information d'application

Indications d'application: This Alexa Fluor® 647-conjugated antibody is suitable for intracellular staining of cell lines and primary cells using BD Cytofix™ Fixation Buffer or BD™ Phosflow Lyse/Fix Buffer. Although this antibody can be used with BD™ Phosflow Perm/Wash Buffer I or BD™ Phosflow Perm Buffers II, III or IV, it performs optimally when used with BD™ Phosflow Perm Buffer II.

Volume d'échantillon: 5 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Références pour anticorps anti-NFKBIA (PE) (ABIN1176898)

Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." dans: Cancer research, Vol. 60, Issue 18, pp. 5303-9, (2000) (PubMed).

Détails sur NFKBIA

Antigène: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Autre désignation: IkappaBalpha (NFKBIA Produits)

Synonymes: anticorps IKBA, anticorps MAD-3, anticorps NFKBI, anticorps AI462015, anticorps Nfkbi, anticorps RL/IF-1, anticorps ECI-6/IKBA, anticorps NFKBIA, anticorps ikbaB, anticorps wu:fb81f08, anticorps zgc:56666, anticorps zgc:77616, anticorps ikbaA, anticorps wu:fj16a07, anticorps zgc:85769, anticorps NFKB inhibitor alpha, anticorps nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha, anticorps nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b, anticorps nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a, anticorps NFKBIA, anticorps Nfkbia, anticorps nfkbiab, anticorps nfkbiaa

Sujet: NF-kappaB is a transcription factor that is a member of the mammalian NF-kappaB/Rel family of proteins. Members of this family are involved in the regulation of cell proliferation, immune function, as well as development. In resting cells, IkappaBalpha binds to and maintains NF-kappaB in the cytoplasm by blocking the nuclear localization sequences of NF-kappaB. In the cellular response to an extracellular signal, IkappaBalpha is phosphorylated and subsequently degraded via the ubiquination-proteasome pathway, allowing NF-kappaB to translocate to the nucleus. Once in the nucleus, NF-kappaB can induce the transcription of IkappaBalpha thereby renewing the cycle so that IkappaBalpha can form a complex with NF-kappaB and maintain it in its cytoplasmic location. IkappaBalpha -/- mice show an increased level of NF-kappaB activity and have been shown to die soon after birth. The 25/IkBa/MAD-3 monoclonal antibody recognizes human IkappaBalpha regardless of phosphorylation status and does not cross-react with mouse IkappaBalpha.
Synonyms: MAD-3, I-kappa-B-alpha, IkB-alpha, NFKBIA, NF-kappa-B inhibitor alpha

Pathways: Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling