STAT6 anticorps (pTyr641) (PE)

N° du produit ABIN1177210

Détail du produit anti-STAT6 anticorps (PE)

Antigène: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Épitope: pTyr641

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp STAT6 est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Stat6 (pY641)

Clone: 18-P

Isotype: IgG2a

Information d'application

Volume d'échantillon: 5 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Références pour anticorps anti-STAT6 (PE) (ABIN1177210)

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." dans: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

Dent, Hu-Li, Paul, Staudt: "T helper type 2 inflammatory disease in the absence of interleukin 4 and transcription factor STAT6." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13823-8, (1998) (PubMed).

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." dans: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, (1996) (PubMed).

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." dans: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, (1996) (PubMed).

Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." dans: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, (1995) (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." dans: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, (1994) (PubMed).

Détails sur STAT6

Antigène: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Autre désignation: STAT6 (STAT6 Produits)

Synonymes: anticorps D12S1644, anticorps IL-4-STAT, anticorps STAT6B, anticorps STAT6C, anticorps signal transducer and activator of transcription 6, anticorps signal transducer and activator of transcription 6-like, anticorps STAT6, anticorps Stat6, anticorps LOC100859543

Sujet: Interleukin-4 (IL-4), a major immunoregulatory cytokine, is secreted by activated T lymphocytes, basophils, and mast cells and plays an important role in modulating T helper cell lineage development. It induces specific gene expression via the tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). Stat6, a member of the signal transducers and activators of transcription protein family, mediates signals for IL-4 and, possibly, IL-13. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following cytokine receptor ligation, Jak kinases are activated and phosphorylate the cytoplasmic tails of the oligomerized receptors. The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. Thus, Stat6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity.
Synonyms: Signal transducer and activator of transcription 6, IL-4 Stat

Poids moléculaire: 100 kDa

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response