CD45 anticorps

N° du produit ABIN119550

Détail du produit anti-CD45 anticorps

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité: Humain, Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Réactivité croisée (Details): Species reactivity (tested):Human, non-Human Primates.

Purification: Affinity Chromatography on Protein A

Immunogène: Human thymocytes and T lymphocytes

Clone: MEM-55

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry (FFPE sections). No pre-treatment of tissue sections is essential.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the original vial at 2-8 °C. Freeze stock solution or aliquots thereof at -20 °C.

Références pour anticorps anti-CD45 (ABIN119550)

de Torre-Minguela, Barberà-Cremades, Gómez, Martín-Sánchez, Pelegrín: "Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process." dans: Scientific reports, Vol. 6, pp. 22586, (2017) (PubMed).

Honda, Yasuda, Tanaka, Hagiwara, Arai, Yoshino: "Functional expression of full-length TrkA in the prokaryotic host Magnetospirillum magneticum AMB-1 by using a magnetosome display system." dans: Applied and environmental microbiology, Vol. 81, Issue 4, pp. 1472-6, (2016) (PubMed).

Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." dans: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).

Möbius-Winkler, Hilberg, Menzel, Golla, Burman, Schuler, Adams: "Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals." dans: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 107, Issue 6, pp. 1943-50, (2010) (PubMed).

Curnis, Gasparri, Sacchi, Cattaneo, Magni, Corti: "Targeted delivery of IFNgamma to tumor vessels uncouples antitumor from counterregulatory mechanisms." dans: Cancer research, Vol. 65, Issue 7, pp. 2906-13, (2005) (PubMed).

Reynaud, Bruno, Boullanger, Grange, Barbesti, Niveleau: "Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies." dans: Cancer letters, Vol. 61, Issue 3, pp. 255-62, (1992) (PubMed).

Détails sur CD45

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: CD45 / LCA (PTPRC Produits)

Synonymes: anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc

Sujet: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

ID gène: 5788

NCBI Accession: NP_002829

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling