CD25 anticorps (PE)

N° du produit ABIN120430

Détail du produit anti-CD25 anticorps (PE)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (1).

Purification: Protein G purified immunoglobulin fraction

Immunogène: Con A stimulated Rat T cells.

Clone: OX-39

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Reconstitute with 1 mL distilled water.

Buffer: PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Date de péremption: 6 months

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 / IL2RA (IL2RA Produits)

Synonymes: anticorps CD25, anticorps IDDM10, anticorps IL2R, anticorps TCGFR, anticorps IL2RAC, anticorps IL-2RA, anticorps Il2r, anticorps Ly-43, anticorps interleukin 2 receptor subunit alpha, anticorps interleukin-2 receptor subunit alpha-like, anticorps interleukin 2 receptor, alpha chain, anticorps IL2RA, anticorps Il2ra, anticorps LOC100222957

Sujet: The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

ID gène: 25704

NCBI Accession: NP_037295

UniProt: P26897

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation