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- Antigène Voir toutes CD28 Anticorps
- CD28
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Reactivité
- Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD28 est conjugé à/à la Biotin
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Application
- Flow Cytometry (FACS)
- Specificité
- Antibody SM268B recognizes a 90 kDa homeodimeric cell surface glycoprotein (CD28). Results of tissue Distribution by Flow Cytometry Analysis: Rat strain: Fischer Cell concentration: 1x10e6 cells per test Antibody concentration used: 1.0 μg/10e6 cells Isotypic control: Biotin Mouse IgG1 Cell source percentage of cells stained above control Thymus: 25.4 % Splenic T Cells*: 73.2 % *(T cells isolated with a Rat T Cell Recovery Column).
- Purification
- Protein G Chromatography
- Immunogène
- Rat CD28 transfected A20J cells
- Clone
- JJ319
- Isotype
- IgG1
- Top Product
- Discover our top product CD28 Anticorps primaire
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Hôte: Lapin Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivité: Humain, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Hôte: Souris Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 Reactivité: Humain, Non-Human Primate FACS Hôte: Souris Monoclonal CD28-2 FITC
anti-CD28 (CD28) (AA 19-152) antibodyCD28 Reactivité: Humain FACS, ELISA Hôte: Lapin Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 Reactivité: Humain, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Hôte: Souris Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivité: Rat FACS, IP Hôte: Souris Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibody (PE)CD28 Reactivité: Rat FACS Hôte: Souris Monoclonal JJ319 PE
anti-CD28 (CD28) antibody (APC)CD28 Reactivité: Humain, Non-Human Primate FACS Hôte: Souris Monoclonal CD28-2 APC
anti-CD28 (CD28) antibody (PE)CD28 Reactivité: Humain, Non-Human Primate FACS Hôte: Souris Monoclonal CD28-2 PE
anti-CD28 (CD28) antibody (PerCP)CD28 Reactivité: Humain, Non-Human Primate FACS Hôte: Souris Monoclonal CD28-2 PerCP
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- Indications d'application
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Flow cytometry (for details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
- Date de péremption
- 6 months
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- Antigène
- CD28
- Autre désignation
- CD28 (CD28 Produits)
- Synonymes
- anticorps Tp44, anticorps CD28RNA, anticorps cd28, anticorps CD28 molecule, anticorps CD28 antigen, anticorps Cd28 molecule, anticorps CD28 molecule L homeolog, anticorps CD28, anticorps Cd28, anticorps cd28.L
- Sujet
- CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat alpha/beta and most gamma/delta T cells, as well as on approximately half of all NK cells.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
- ID gène
- 25660
- NCBI Accession
- NP_037253
- UniProt
- P31042
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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