CD4 anticorps
-
- Antigène Voir toutes CD4 Anticorps
- CD4
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp CD4 est non-conjugé
-
Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
- Specificité
- The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes.
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatography on Protein A
- Immunogène
- Human thymocytes and T lymphocytes
- Clone
- MEM-115
- Isotype
- IgG2a
- Top Product
- Discover our top product CD4 Anticorps primaire
-
-
- Indications d'application
-
Excellent for Immunoprecipitation. Functional The antibody MEM-115 blocks binding of HIV gp120 to CD4 Molecule and it also strongly inhibits CD4-MHC Class II interactions. Flow Cytometry: Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells. MEM-115 is negative in Western blotting even with non-reduced samples of cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 without preservatives
- Agent conservateur
- Without preservative
- Stock
- 4 °C/-20 °C
- Stockage commentaire
-
Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: One year from despatch. - Date de péremption
- 12 months
-
-
The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." dans: EMBO reports, Vol. 5, Issue 9, pp. 883-8, (2004) (PubMed).
: "Cell surface receptors, virus entry and tropism of primate lentiviruses." dans: The Journal of general virology, Vol. 83, Issue Pt 8, pp. 1809-29, (2002) (PubMed).
: "p56Lck anchors CD4 to distinct microdomains on microvilli." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 2008-13, (2002) (PubMed).
: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." dans: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
: "
-
The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." dans: EMBO reports, Vol. 5, Issue 9, pp. 883-8, (2004) (PubMed).
-
- Antigène
- CD4
- Autre désignation
- CD4 (CD4 Produits)
- Synonymes
- anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
- Sujet
- CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- ID gène
- 920
- UniProt
- P01730
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
-