PRKACA anticorps (Catalytic Subunit)

N° du produit ABIN127012

Cet anticorps Lapin Polyclonal détecte spécifiquement PRKACA dans WB et IP. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Mouton et Porc.

Aperçu rapide pour PRKACA anticorps (Catalytic Subunit) (ABIN127012)

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mouton, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKACA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-PRKACA anticorps

Épitope: Catalytic Subunit

Specificité: Recognizes a 40 kDa PKA on SDS-PAGE immunoblots

Purification: Affinity purified

Immunogène: Synthetic peptide surrounding amino acid 11 of the catalytic subunit of PKA alpha isoform.

Information d'application

Indications d'application: Western blot: 0.5-4 μg/mL, Jukat cell lysate can be used as a positive control. Immunoprecipitation: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Détails sur PRKACA

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Autre désignation: PRKACA

Sujet: Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

ID gène: 5566

NCBI Accession: NP_002721

UniProt: P17612

Pathways: Signalisation NF-kappaB, Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2