IGF1R anticorps (pTyr1161)

N° du produit ABIN1355850

Détail du produit anti-IGF1R anticorps

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Épitope: pTyr1161

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IGF1R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: The antibody detects endogenous level of IGF-1R only when phosphorylated at tyrosine 1161.

Immunogène: Peptide sequence around phosphorylation site of pTyr1161 (D-I-Y (p) -E-T) derived from Human IGF-1R. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C

Détails sur IGF1R

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Autre désignation: IGF-1R (IGF1R Produits)

Synonymes: anticorps CMT2N, anticorps CD221, anticorps IGFIR, anticorps IGFR, anticorps JTK13, anticorps IGFIRC, anticorps IGFR1, anticorps IGF-1R, anticorps IGF1R, anticorps DKFZp469I0618, anticorps igf-1r, anticorps xIGF-1R, anticorps xIGFR, anticorps xigf1r, anticorps A330103N21Rik, anticorps D930020L01, anticorps hyft, anticorps igf-ir, anticorps IGF-IR, anticorps IGF-IRb, anticorps wu:fb03h06, anticorps IGF-IRa, anticorps sb:eu1090, anticorps wu:fi20b10, anticorps alanyl-tRNA synthetase, anticorps insulin like growth factor 1 receptor, anticorps insulin-like growth factor 1 receptor, anticorps insulin-like growth factor 1, anticorps insulin-like growth factor I receptor, anticorps insulin like growth factor 1 receptor S homeolog, anticorps insulin-like growth factor 1b receptor, anticorps insulin-like growth factor 1a receptor, anticorps AARS, anticorps IGF1R, anticorps Igf1r, anticorps igf1r, anticorps Igf1, anticorps LOC780503, anticorps LOC100136427, anticorps igf1r.S, anticorps igf1rb, anticorps igf1ra

Sujet:  Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.When present in a hybrid receptor with INSR, binds IGF1.shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. 

Poids moléculaire: 200 kDa

NCBI Accession: NP_000866, NM_000875

UniProt: P08069

Pathways: Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy