WNT1 anticorps
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- Antigène Voir toutes WNT1 Anticorps
- WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp WNT1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificité
- The antibody recognizes Human WNT-1. Other species not tested.
- Purification
- Affinity chromatography employing immobilized hWNT-1 matrix
- Immunogène
- Highly pure (>98%) recombinant human WNT-1
- Top Product
- Discover our top product WNT1 Anticorps primaire
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- Indications d'application
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ElisaIndirect: To detect hWNT-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hWNT-1. Sandwich: To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human WNT-1 (PP1231B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western BlotTo detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- Date de péremption
- 12 months
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- Antigène
- WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))
- Autre désignation
- WNT1 (WNT1 Produits)
- Synonymes
- anticorps Xint-1, anticorps Xwnt1, anticorps int-1, anticorps int1, anticorps wnt-1, anticorps wnt1-a, anticorps Int-1, anticorps Wnt-1, anticorps sw, anticorps swaying, anticorps BMND16, anticorps INT1, anticorps OI15, anticorps Int1, anticorps WNT-1, anticorps sb:eu647, anticorps zgc:194464, anticorps zgc:194478, anticorps WNT1, anticorps Wnt family member 1, anticorps Wnt family member 1 L homeolog, anticorps wingless-type MMTV integration site family, member 1, anticorps WNT1, anticorps wnt1.L, anticorps Wnt1, anticorps wnt1
- Sujet
- Synonyms: INT-1, INT1, Proto-oncogene protein Wnt-1
- ID gène
- 7471
- NCBI Accession
- NP_005421
- UniProt
- P04628
- Pathways
- Signalisation WNT, Dopaminergic Neurogenesis
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