PRKAA1 anticorps (AA 500-550)

N° du produit ABIN1574209

Détail du produit anti-PRKAA1 anticorps

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Épitope: AA 500-550

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAA1 est non-conjugé

Application: Western Blotting (WB)

Specificité: AMPKalpha1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKalpha1 and does not cross-react with AMPKalpha2. It is predicted to react with mouse AMPKalpha1 according to sequence homology. Positive Control: HEK293 and MCF-7

Réactivité croisée (Details): AMPKα1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKα1 and does not cross-react with AMPKα2. It is predicted to react with mouse AMPKα1 according to sequence homology.Positive Control: HEK293 and MCF-7

Purification: Immunoaffinity chromatography

Immunogène: KLH-coupled synthetic peptide within AA 500–550 of human AMPKα1

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mL Other Applications: User optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détails sur PRKAA1

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Autre désignation: AMPKalpha1 (PRKAA1 Produits)

Synonymes: anticorps AMPK, anticorps AMPKa1, anticorps cb116, anticorps im:7154392, anticorps sb:cb130, anticorps wu:fa94c10, anticorps AMPK1, anticorps AMPKalpha1, anticorps AI194361, anticorps AI450832, anticorps AL024255, anticorps C130083N04Rik, anticorps ampk, anticorps ampka1, anticorps PRKAA1, anticorps protein kinase AMP-activated catalytic subunit alpha 1, anticorps protein kinase, AMP-activated, alpha 1 catalytic subunit, anticorps protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog, anticorps PRKAA1, anticorps prkaa1, anticorps Prkaa1, anticorps prkaa1.L

Sujet: AMP-activated protein kinase (AMPK) plays an important role in the regulation of energy homeostasis. It is activated by an elevated AMP/ATP ratio caused by cellular and environmental stress, such as heat shock, hypoxia, and ischemia. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3). Phosphorylation of AMPKalpha at Thr172 is the major regulatory site which is required for AMPK activation. AMPKalpha1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 50050 of human AMPKalpha1 (Swiss Prot: Q13131).

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg