alpha Adaptin anticorps (AA 706-727)

N° du produit ABIN1804775

Détail du produit anti-alpha Adaptin anticorps

Antigène: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Épitope: AA 706-727

Reactivité: Humain, Souris, Chien, Porc, Cheval, Lapin, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha Adaptin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human AP2A1 / AP2-Alpha. This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-ELEPPAPESPMALLADPAPAAD from an internal region of human AP2A1 / AP2-Alpha (NP_055018.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Ferret, Dog, Cat, Panda, Horse, Rabbit, Pig (100%), Rat (95%), Opossum, Guinea pig (91%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 μg/mL. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur alpha Adaptin

Antigène: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Autre désignation: AP2A1 / AP2-Alpha (AP2A1 Produits)

Synonymes: anticorps ADTAA, anticorps AP2-ALPHA, anticorps CLAPA1, anticorps adtaa, anticorps clapa1, anticorps ap2-alpha, anticorps AP2A1, anticorps Adtaa, anticorps adaptor related protein complex 2 alpha 1 subunit, anticorps adaptor-related protein complex 2, alpha 1 subunit, anticorps AP-2 complex subunit alpha-1, anticorps AP2A1, anticorps ap2a1, anticorps Ap2a1, anticorps EDI_093420, anticorps EDI_259990

Sujet: Name/Gene ID: AP2A1

Synonyms: AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP-2 complex subunit alpha-1, AP2 Alpha, CLAPA1, AP2-alpha

ID gène: 160

NCBI Accession: NP_055018

UniProt: O95782

Pathways: Signalisation Notch, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation