IL-6 anticorps

N° du produit ABIN181728

L’anticorps Lapin Polyclonal anti-IL-6 a été validé pour WB, IF, IHC (p), EIA et Func. Il convient pour détecter IL-6 dans des échantillons de Humain.

Aperçu rapide pour IL-6 anticorps (ABIN181728)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-6 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-IL-6 anticorps

Purification: Affinity Chromatography employing immobilized hIL-6 matrix

Immunogène: Highly pure (>98%) E.coli derived recombinant Human Interleukin 6.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-6 (1.0 ng/mL), a concentration of 0.20 - 0.40 μg/mL of this antibody is required. ELISA: Indirect: To detect hIL-6 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich: To detect hIL-6 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIL-6 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-6. Western blot: To detect hIL-6 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IL-6

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: Interleukin-6 (IL6)

Sujet: Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2

ID gène: 9606

UniProt: P05231

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome