F4/80 anticorps

N° du produit ABIN181736

Détail du produit anti-F4/80 anticorps

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody is useful for the detection of major subpopulations of resident tissue macrophages. The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This clone BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to ß-cell destruction and diabetes in a mouse diabetes model. Antigen Distribution on Tissue and Isolated Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells. The antigen is expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.Antigen Distribution on Tissue and Isolated Sections:

Purification: Immunoaffinity Chromatography

Immunogène: Cultured macrophages. The F4/80 antigen is a 125 kD extracellular membrane protein sensitive to 2-Mercaptoethanol.

Clone: BM8

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 0.4 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Mouse spleen. Has been described to work in FACS.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer’s hemalum

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.05 % (v/v) Kathon CG as a preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the lyophilised antibody at 2-8 °C. After reconstitution add a conserving agent (e.g. thimerosal) and store undiluted at 2-8 °C.

Références pour anticorps anti-F4/80 (ABIN181736)

Stangl, Gehrmann, Riegger, Kuhs, Riederer, Sievert, Hube, Mocikat, Dressel, Kremmer, Pockley, Friedrich, Vigh, Skerra, Multhoff: "Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 2, pp. 733-8, (2011) (PubMed).

Spyridonidou, Fousteris, Antonia, Chatzianastasiou, Papapetropoulos, Nikolaropoulos: "Tricyclic indole and dihydroindole derivatives as new inhibitors of soluble guanylate cyclase." dans: Bioorganic & medicinal chemistry letters, Vol. 19, Issue 16, pp. 4810-3, (2009) (PubMed).

Kimura, Miyashita, Suzuki, Kobayashi, Watanabe, Sonoda, Ohta, Fujiwara, Shimosegawa, Sato: "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis." dans: Blood, Vol. 113, Issue 19, pp. 4810-8, (2009) (PubMed).

Kakehashi, Nishioku, Tsukuba, Kadowaki, Nakamura, Yamamoto: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).

Torzewski, Shaw, Han, Shortal, Lackner, Witztum, Palinski, Tsimikas: "Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 12, pp. 2307-12, (2004) (PubMed).

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Autre désignation: Macrophage F4/80 Antigen (EMR1 Produits)

Synonymes: anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1

Sujet: F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

ID gène: 13733

NCBI Accession: NP_034260

UniProt: Q61549