GADD34 anticorps (C-Term)

N° du produit ABIN184571

Détail du produit anti-GADD34 anticorps

Antigène: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GADD34 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: PPP1R15A / GADD34

Séquence: AAALDLSGRR G

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.3.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4 kDa according to NP_055145.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kel

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GADD34

Antigène: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Autre désignation: PPP1R15A (PPP1R15A Produits)

Synonymes: anticorps 9630030H21, anticorps Gadd34, anticorps Myd116, anticorps GADD34, anticorps Peg-3, anticorps RGD1624209, anticorps protein phosphatase 1 regulatory subunit 15A, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 15A, anticorps protein phosphatase 1, regulatory subunit 15A, anticorps PPP1R15A, anticorps Ppp1r15a

Sujet: PPP1R15A, GADD34, protein phosphatase 1, regulatory (inhibitor) subunit 15A, growth arrest and DNA-damage-inducible 34, protein phosphatase 1, regulatory subunit 15A

ID gène: 23645

NCBI Accession: NP_055145

Pathways: ER-Nucleus Signaling, Unfolded Protein Response