Caspase 3 anticorps (AA 29-175) (FITC)

N° du produit ABIN1858244

Détail du produit anti-Caspase 3 anticorps (FITC)

Antigène: Caspase 3 (CASP3)

Épitope: AA 29-175

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 3 est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Séquence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGLCIII NNKNFHKSTGMSARNGTDVD AANLRETFMA LKYEVRNKND LTREEIMELM DSVSKEDHSK RSSFVCVILS HGDEGVIFGT NGPVDLKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD

Specificité: It has been selected for its ability to recognize CASP3 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant CASP3 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CASP3 conjugated to fitc.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant CASP3 (Ser29~Asp175) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Références pour anticorps anti-Caspase 3 (FITC) (ABIN1858244)

Chen, Cao, Asare, Lv, Zhu, Li, Wei, Gao, Zhang, Mao, Gao, Fan: "Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction by danhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms." dans: Journal of ethnopharmacology, Vol. 194, pp. 559-570, (2016) (PubMed).

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase 3 (CASP3 Produits)

Synonymes: anticorps CPP32, anticorps CPP32B, anticorps SCA-1, anticorps A830040C14Rik, anticorps AC-3, anticorps Apopain, anticorps CC3, anticorps Caspase-3, anticorps Lice, anticorps Yama, anticorps mldy, anticorps xcpp32, anticorps casp3, anticorps zgc:100890, anticorps CASP-3, anticorps caspase-3, anticorps caspase 3, anticorps caspase 3 S homeolog, anticorps caspase 3, apoptosis-related cysteine peptidase a, anticorps caspase 3, apoptosis-related cysteine peptidase, anticorps CASP3, anticorps Casp3, anticorps casp3.S, anticorps casp3a

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation