FAS anticorps (Biotin)
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- Antigène Voir toutes FAS Anticorps
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp FAS est conjugé à/à la Biotin
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Application
- Flow Cytometry (FACS)
- Attributs du produit
- The 4ADX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily.
- Clone
- 4ADX2
- Isotype
- IgG1
- Top Product
- Discover our top product FAS Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
- Repeat step 5.
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- Autre désignation
- CD95 (FAS Produits)
- Synonymes
- anticorps ALPS1A, anticorps APO-1, anticorps APT1, anticorps CD95, anticorps FAS1, anticorps FASTM, anticorps TNFRSF6, anticorps AI196731, anticorps APO1, anticorps TNFR6, anticorps Tnfrsf6, anticorps lpr, anticorps Fas cell surface death receptor, anticorps Fas (TNF receptor superfamily member 6), anticorps FAS, anticorps Fas, anticorps fas
- Sujet
- CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance.
- Pathways
- Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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