MME anticorps (FITC)

N° du produit ABIN2144484

Détail du produit anti-MME anticorps (FITC)

Antigène: MME (Membrane Metallo-Endopeptidase (MME))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MME est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: 4ACB-CALLA reacts with CD10, 100 kDa common acute lymphoblastic leukemia antigen (CALLA), which is expressed on lymphoid precursors, germinal center B cells, and peripheral blood granulocytes. CD10 is a regulator of B cell growth and proliferation. CD10 antibody is used in conjunction with other reagents in the phenotyping of leukemia.*CD10 is a phenotyping marker of leukemic cells that may have prognostic significance in leukemia clinical application.

Clone: 4ACB-CALLA

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur MME

Antigène: MME (Membrane Metallo-Endopeptidase (MME))

Autre désignation: CD10 (MME Produits)

Synonymes: anticorps CALLA, anticorps CD10, anticorps NEP, anticorps SFE, anticorps 6030454K05Rik, anticorps C85356, anticorps Nep, anticorps neprilysin, anticorps membrane metalloendopeptidase, anticorps membrane metallo endopeptidase, anticorps membrane metallo-endopeptidase, anticorps MME, anticorps Mme

Classe de substances: Chemical

Pathways: Signalisation RTK, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration