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- Antigène Voir toutes CD4 Anticorps
- CD4
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD4 est conjugé à/à la APC
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Application
- Flow Cytometry (FACS)
- Attributs du produit
- The 4AGK1.5 monoclonal antibody reacts with the mouse CD4 Molecule, a 55 kDa cell surface receptor. It is a member of the lg superfamily,primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC classII and associating with the protein tyrosine kinase, lck.
- Isotype
- IgG2b
- Top Product
- Discover our top product CD4 Anticorps primaire
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivité: Humain, Souris, Rat, Poulet, Porc FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivité: Humain, Souris, Rat, Poulet, Singe, Porc, Chien, Cat, Lapin, Boeuf (Vache), Mouton, Cobaye, Chévre, Cheval, Avian, Âne, Hamster WB Hôte: Chèvre Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 Reactivité: Humain FACS, WB, CyTOF Hôte: Souris Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) (AA 300-400) antibodyCD4 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivité: Souris FACS, IP, IHC (fro), ICC, Func Hôte: Rat Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 Reactivité: Humain FACS Hôte: Souris Monoclonal MEM-241 FITC
anti-CD4 (CD4) (AA 301-400) antibodyCD4 Reactivité: Humain, Souris, Rat FACS, WB, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 Reactivité: Humain FACS Hôte: Souris Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 Reactivité: Rat FACS Hôte: Souris Monoclonal OX-35 PE
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL a certain amount of prepared cells and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.3-0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined in two hours on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD4
- Autre désignation
- CD4 (CD4 Produits)
- Synonymes
- anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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