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IL-6 anticorps

Cet anticorps anti-IL-6 est un anticorps Rat Monoclonal détectant IL-6 dans WB, ELISA, FACS et IF. Adapté pour Souris.
N° du produit ABIN218142

Aperçu rapide pour IL-6 anticorps (ABIN218142)

Antigène

Voir toutes IL-6 (IL6) Anticorps
IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité

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Souris

Hôte

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Rat

Clonalité

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Monoclonal

Conjugué

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Cet anticorp IL-6 est non-conjugé

Application

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
  • Specificité

    Recognizes mouse Interleukin-6 (IL-6).

    Purification

    Purified

    Immunogène

    COS-7-expressed recombinant mouse IL-6.

    Isotype

    IgG1 kappa
  • Indications d'application

    Approved: ELISA, Flo, IF, WB

    Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-6 protein levels.3-5 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-6-positive cells within mixed cell populations.6 Recombinant mouse IL-6 or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions.

    Commentaires

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    100 mM borate buffered saline,  pH 8.0. No preservatives or amine-containing buffer salts added.

    Agent conservateur

    Without preservative

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Antigène

    IL-6 (IL6) (Interleukin 6 (IL6))

    Autre désignation

    IL6

    Sujet

    Name/Gene ID: IL6
    Family: Hormone

    Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

    ID gène

    3569

    UniProt

    P05231

    Pathways

    Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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