TNFRSF1A anticorps

N° du produit ABIN2192161

Détail du produit anti-TNFRSF1A anticorps

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNFRSF1A est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunoassay (IA)

Réactivité croisée (Details): Cross reactivity: Mouse TNF-RII : Yes, Mouse TNF-alpha Minimal crossreactivity problematic in case of TNF-RI knockout studies and when used in high concentrations in functional studies

Stérilité: 0.2 μm filtered

Isotype: IgG

Information d'application

Indications d'application: For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Antibody reactivity with cell bound or soluble TNF-RI is not inhibited by high concentrations of mouse TNFα.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-TNFRSF1A (ABIN2192161)

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." dans: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, (2000) (PubMed).

Lucas, Garcia, Donati, Hribar, Mandriota, Giroud, Buurman, Fransen, Suter, Nunez, Pepper, Grau: "Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells." dans: European journal of immunology, Vol. 28, Issue 11, pp. 3577-86, (1998) (PubMed).

Bemelmans, Gouma, Buurman: "LPS-induced sTNF-receptor release in vivo in a murine model. Investigation of the role of tumor necrosis factor, IL-1, leukemia inhibiting factor, and IFN-gamma." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5554-62, (1993) (PubMed).

Détails sur TNFRSF1A

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: Cd120a, Tnf-Ri (TNFRSF1A Produits)

Synonymes: anticorps CD120a, anticorps FPF, anticorps MS5, anticorps TBP1, anticorps TNF-R, anticorps TNF-R-I, anticorps TNF-R55, anticorps TNFAR, anticorps TNFR1, anticorps TNFR1-d2, anticorps TNFR55, anticorps TNFR60, anticorps p55, anticorps p55-R, anticorps p60, anticorps TNF-R1, anticorps TNF-alphaR1, anticorps TNFRI, anticorps TNFRp55, anticorps TNFalpha-R1, anticorps Tnfr-2, anticorps Tnfr1, anticorps fpf, anticorps tbp1, anticorps p55-r, anticorps tnf-r, anticorps tnfar, anticorps tnfr1, anticorps cd120a, anticorps tnfr55, anticorps tnfr60, anticorps xtnfr1, anticorps wu:fj65b10, anticorps zgc:85914, anticorps TNFR I, anticorps TNFRSF1A, anticorps TNFA, anticorps cTNF, anticorps TNF receptor superfamily member 1A, anticorps tumor necrosis factor receptor superfamily, member 1a, anticorps TNF receptor superfamily member 1A S homeolog, anticorps tumor necrosis factor, anticorps TNFRSF1A, anticorps Tnfrsf1a, anticorps tnfrsf1a.S, anticorps tnfrsf1a, anticorps TNF

Sujet: The polyclonal antibody recognizes the extracellular part of the mouse Tumor Necrosis Factor Receptor type 1 (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways. Aliases Tumor necrosis factor receptor superfamily member 1A, TNF-RI, p55/p60

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway