PKC alpha anticorps (pSer657, Tyr658)

N° du produit ABIN222045

Cet anticorps anti-PKC alpha est un anticorps Lapin Polyclonal détectant PKC alpha dans WB et ELISA. Adapté pour Humain, Rat et Lapin.

Aperçu rapide pour PKC alpha anticorps (pSer657, Tyr658) (ABIN222045)

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivité: Humain, Rat, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC alpha est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-PKC alpha anticorps

Épitope: pSer657, Tyr658

Specificité: Detects an 82kD protein corresponding to the molecular mass of phosphorylated PKCa on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were seen in other PKCa-expressing cells, such as human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb, but not other PKC isoforms in rat brain lysate.

Purification: Immunoaffinity purified

Immunogène: Phospho-PKCa (Ser-657/Tyr-658) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKCg, although this reactivity has not been confirmed.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA, WB

Usage: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur PKC alpha

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Autre désignation: PRKCA / PKC-Alpha

Sujet: Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha

ID gène: 5578

UniProt: P17252

Pathways: Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling