PCNA anticorps

N° du produit ABIN233602

Détail du produit anti-PCNA anticorps

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificité: Recognizes a non-histone protein of 36kD, proliferating cell nuclear antigen (PCNA). Species cross-reactivity: Human, mouse, rat, insect and yeast.

Purification: Protein A purified

Immunogène: Recombinant rat PCNA protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: Flo, IHC, IHC-P, IP, WB (1:25)

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate. Use Protein A. Western Blot: 1:25 for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. Requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Raji cells. Tonsil, lymph node, or small intestine.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, glycerol to 40 %.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA / Cyclin (PCNA Produits)

Synonymes: anticorps PCNAR, anticorps Pcna/cyclin, anticorps pcna, anticorps AO090003000846, anticorps 53/13, anticorps CG9193, anticorps DmPCNA, anticorps Dmel\\CG9193, anticorps MUS209, anticorps Mus209, anticorps PCNA, anticorps PCNA/mus209, anticorps Pcna, anticorps dPCNA, anticorps l(2)02448, anticorps l(2)56F, anticorps l(2)56Fa, anticorps l(2)s1534, anticorps cb16, anticorps fb36g03, anticorps wu:fa28e03, anticorps wu:fb36g03, anticorps pcna-A, anticorps proliferating cell nuclear antigen, anticorps DNA polymerase III sliding clamp, anticorps Proliferating cell nuclear antigen, anticorps Proliferating cell nuclear antigen-like, anticorps proliferating cell nuclear antigen L homeolog, anticorps proliferating cell nuclear antigen 1, anticorps PCNA, anticorps Pcna, anticorps pcna, anticorps MMP_RS08810, anticorps ANI_1_512014, anticorps AOR_1_1500154, anticorps MGC53867, anticorps pcna.L, anticorps pcna1, anticorps LOC106420145, anticorps pcn1

Sujet: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy