UBA3 anticorps

N° du produit ABIN2463920

Détail du produit anti-UBA3 anticorps

Antigène: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBA3 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBA3.

Information d'application

Indications d'application: UBA3 antibody can be used for detection of UBA3 by ELISA at 1:1562500. UBA3 antibody can be used for detection of UBA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store UBA3 antibody at -20 °C.

Détails sur UBA3

Antigène: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Autre désignation: UBA3 (UBA3 Produits)

Synonymes: anticorps UBE1C, anticorps hUBA3, anticorps Ube1c, anticorps ube1c, anticorps wu:fb75e04, anticorps wu:fc37b11, anticorps zgc:55528, anticorps A830034N06Rik, anticorps AI256736, anticorps AI848246, anticorps AW546539, anticorps ubiquitin like modifier activating enzyme 3, anticorps ubiquitin-like modifier activating enzyme 3, anticorps ubiquitin like modifier activating enzyme 3 S homeolog, anticorps UBA3, anticorps Uba3, anticorps uba3, anticorps uba3.S

Sujet: UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Poids moléculaire: 52 kDa

ID gène: 9039

NCBI Accession: NP_003959

UniProt: Q8TBC4