This antibody is expected to recognize both the human SART1(259) and SART1(800) proteins.
Purification
SART1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
SART1 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of SART1.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 125 kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
SART1
(Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))