Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
VPS25 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
VPS25 antibody was raised against a 10 amino acid synthetic peptide near the internal region of VPS25.
VPS25
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 20-22 kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7 kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
VPS25
(Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))