Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
S100A9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
S100A9 antibody was raised against a 15 amino acid synthetic peptide near the internal region of S100A9.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 16 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 13.2 kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15 kDa ) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280 (50) :41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows nuclear staining in select cells, Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.