Cyclin E1 anticorps

N° du produit ABIN265380

Détail du produit anti-Cyclin E1 anticorps

Antigène: Cyclin E1 (CCNE1)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin E1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody detects endogenous levels of Cyclin E1 protein (region surrounding Leu389).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Cyclin E1

Antigène: Cyclin E1 (CCNE1)

Autre désignation: Cyclin E1 (CCNE1 Produits)

Synonymes: anticorps CCNE1, anticorps ccne, anticorps cyclinE, anticorps CCNE, anticorps AW538188, anticorps CycE1, anticorps CYCLE, anticorps Ccne, anticorps cyce2, anticorps cycE3, anticorps cyce, anticorps fa18e07, anticorps wu:fa18e07, anticorps cyclin E1, anticorps cyclin E1 L homeolog, anticorps cyclin E1 S homeolog, anticorps CCNE1, anticorps ccne1, anticorps Ccne1, anticorps ccne1.L, anticorps ccne1.S

Sujet: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.Synonyms: CCNE, CCNE1, Cyclin-E1

Poids moléculaire: approx. 48 kDa

ID gène: 898

NCBI Accession: NP_001229

UniProt: P24864

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases