NFKBIE anticorps

N° du produit ABIN265484

Détail du produit anti-NFKBIE anticorps

Antigène: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFKBIE est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of IkappaB-epsilon protein. (region surrounding Asp17)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur NFKBIE

Antigène: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Autre désignation: NFKBIE / IKBE (NFKBIE Produits)

Synonymes: anticorps NFKBIE, anticorps IKBE, anticorps Slc35b2, anticorps NFKB inhibitor epsilon, anticorps nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon, anticorps nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon, anticorps NFKBIE, anticorps Nfkbie

Sujet: The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation Serines 32 and 36. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.Synonyms: I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon, NF-kappa-B inhibitor epsilon

Poids moléculaire: approx. 45 kDa

ID gène: 4794

NCBI Accession: NP_004547

UniProt: O00221

Pathways: Signalisation NF-kappaB, Maintenance of Protein Location