Histone 3 anticorps (H3K9me3)
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- Antigène Voir toutes Histone 3 (H3) Anticorps
- Histone 3 (H3)
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Épitope
- H3K9me3
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Histone 3 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC)
- Purification
- Protein A Chromatography
- Immunogène
- This Histone H3 trimethylLys9 antibody was raised against a peptide including trimethyllysine 9 of histone H3.
- Isotype
- IgG
- Top Product
- Discover our top product H3 Anticorps primaire
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- Indications d'application
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Recommended antibody dilutions for different applications:
ChIP: 10 µg for each ChIP
ICC/IF: 1 µg/mL dilution
WB: 0.5 - 2 µg/mL dilution
CUT&Tag: 1 µg for each 50 µL reaction
Optimal working dilution should be determined by the investigator. - Restrictions
- For Research Use only
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- Concentration
- 1 μg/μL
- Buffer
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Stock
- -20 °C
- Stockage commentaire
- Antibodies in solution can be stored at -20 °C for 2 years.
- Date de péremption
- 6 months
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H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." dans: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
: "The methyltransferase Suv39h1 links the SUMO pathway to HP1α marking at pericentric heterochromatin." dans: Nature communications, Vol. 7, pp. 12224, (2016) (PubMed).
: "Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle." dans: The Journal of clinical investigation, Vol. 125, Issue 12, pp. 4463-82, (2015) (PubMed).
: "Induction of sarcomas by mutant IDH2." dans: Genes & development, Vol. 27, Issue 18, pp. 1986-98, (2013) (PubMed).
: "Chromatin changes in dicer-deficient mouse embryonic stem cells in response to retinoic acid induced differentiation." dans: PLoS ONE, Vol. 8, Issue 9, pp. e74556, (2013) (PubMed).
: "Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is susceptible to Brd4 inhibition." dans: Genes & development, Vol. 27, Issue 18, pp. 1974-85, (2013) (PubMed).
: "Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation." dans: Nature structural & molecular biology, Vol. 19, Issue 11, pp. 1155-60, (2012) (PubMed).
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H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." dans: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
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- Antigène
- Histone 3 (H3)
- Autre désignation
- Histone H3 (H3 Produits)
- Synonymes
- anticorps H-3, anticorps histocompatibility 3, anticorps H3
- Poids moléculaire
- 17 kDa
- ID gène
- 3020
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